Anggraini Barlian
The aging process that can trigger the emergence of degenerative diseases is caused by oxidative stress conditions. Oxidative stress occurs because of the imbalance of free radical manifestations caused by the formation of ROS (reactive oxygen species). Free radical imbalances in these cells resulted in toxic accumulated the results of the reaction of cellular redox are disrupted and can be harmful to important components in cells. One way that is currently developed in skin therapy is through a physical approach, such as photodynamic therapy (PDT). PDT is a therapeutic procedure using photocessitation compounds. In the medical world, the PDT application has been widely used for Cancer pre-skin therapy, also on damage to the skin due to sunlight. The compound of photosensitations used is ZN-TBP compounds that are reactive to convert oxygen to free radicals and Oxygen Singlets so as to be able to increase oxidative stress which is indicated by increasing ROS in cells. Whereas the photoprotective compound used is nano-propolis which has a polyphenol and flavonoid content can describe the reaction of the lipid chain, inhibit the reaction of cheluminescence, and can describe ROS. The purpose of this research is to study the effect effects of propolist photoprotective compounds for anti-aging agents in normal human skin cell cultures induced by oxidative stress through the influence of photoabsorption intensity by photosensititation compounds in the PDT application. Calculation of this intensity is based on the electronic structure of the basic state of the photocessitation molecule and biological analysis through ROS regulation in skin cell culture. Research will be carried out in two approaches which are a combination of experimental and computing investigations. The experimental investigation was conducted to test the efficiency of photosensitational compounds in the form of Nano-Material ZN-TBP and photoprotective compounds from nano-propolis in the PDT process in normal human skin cell cultures for anti-aging modeling on human skin. Experiments are carried out in vitro by using human dermis fibroblast cell culture to see more on the cellular response that occurs in the process of skin aging and the effect of PDT, and quantitative analysis through ROS measurements with ROS Fluorimetric Assay. Computational investigations are carried out to verify and validate understanding at atomic levels through simulation and computing devices that support experimental investigations. Through the combination of these two methods is expected to provide expectations in the accuracy of the results of this study. For calculating the photobsorption intensity by ZN-TBP, we will use the power of the power dipole perturbation approach and modeled with polarizable-continuum models (PCM), the calculation will use several alternatives such as Quantum Expresso and VASP, which will be adjusted to the computing performance capabilities in the Advanced Computing Laboratory Physics.